HaCaT

Skin

Male

Keratinocyte

1

Aneuploid (hypotetraploid)

10% FBS, 2 mM L-glutamine, 4.5 g/L glucose

Remove the culture medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add freshly prepared 0.05% EDTA solution, 1-2ml per T25, 2.5ml per T75 cell culture flask, the cell sheet must be covered completely, and incubate at 37 ?C for 10 min. Add freshly prepared trypsin/EDTA, 0.05%/0.025% solution *2 (1ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. The cells must detach within 1-2 minutes. Stop the trypsin activity using FBS-containing cell culture medium. Dispense into new flasks which contain fresh cell culture medium.

1 x 10^4 cells/cm^2

CM-1 (Catalog number CLS-800100)

Deutsch:
Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen.
Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.