Recombinant Human Tissue Plasminogen Activator

Tissue plasminogen activator (abbreviated PLAT or tPA) is a secreted serine proteasewhich converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Recombinant Human Tissue Plasminogen Activator produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a MW = 59 kDa. The tPA is purified by proprietary chromatographic techniques.

Lyophilized from a sterile filtered solution. Each mg of TPA contains 1.7 g L-arginine + 0.5 g phosphoric acid, and 4 mg Tween-80.

Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.