Vergleich

Recombinant Human IL-2 HQ

ArtNr CS-CRI254A
Hersteller Cell Sciences
Menge 10 ug
Kategorie
Typ Proteins
Specific against Human
Purity >95% by SDS-PAGE
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products T cell growth factor, aldesleukin, interleukin-2, involved in regulation of T-cell clonal expansion
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Biological Activity
Bioactivity is assayed by measuring the dose dependent effect of human IL-2 on proliferation of CTLL-2 cells. The EC50 for a typical batch of recombinant human IL-2 is 1.08 ng/ml.
Description
Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL-2 may be of use as an anti-tumor agent in cancer therapy. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity. The Recombinant Human IL-2 HQ contains 133 amino acids and a 16 a.a. histidine based tag for a total lenght of 149 a.a.
Endotoxin Level
<0.005 ng per ug of product (0.05 EU/ug) as measured by turbidimetric kinetic assay.
Formulation
Lyophilized from a 0.2 um sterile filtered solution of PBS, pH 7.2.
Molecular Weight
Predicted MW = 17.5 kDa. The recombinant protein migrates with an apparent MW = 22 kDa in SDS-PAGE.
Reconstitution
Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The optimal concentration should be determined by the user for each specific application.
Storage and Stability
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20C to -80C. Reconstituted protein should be used immediately or stored in working aliquots at -20C to -80C. Avoid repeated freeze-thaw cycles.
Specificity
>1 x 10E6 U/mg

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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Lieferung vsl. bis 30.08.2024 

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