Recombinant Human HIF-1 alpha

Recombinant Human HIF-1 alpha is a single, non-glycosylated polypeptide chain containing 298 amino acids (aa 530-826). Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that responds to changes in available oxygen in the cellular environment, specifically, to decreases in oxygen, or hypoxia. It belongs to the PER-ARNT-SIM (PAS) subfamily of the basic-helix-loop-helix (bHLH) family of transcription factors and is a heterodimer composed of alpha and beta subunits. Under hypoxic conditions, HIF-1 alpha activates the transcription of more than 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, VEGF, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 alpha also plays a crucial role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.

Sterile filtered liquid solution in 20 mM Tris-HCl, pH 7.5, +1 mM DTT.

Stable for 1 month at 2-4C. For longer storage, aliquot and freeze at -80C. Avoid repeated freeze-thaw cycles.