Vergleich

Mouse Anti-Human NEFM Clone NF-09 mAb

ArtNr CS-CSI14450
Hersteller Cell Sciences
Menge 25 ug
Kategorie
Typ Antibody Monoclonal
Applikationen WB, IHC
Clon NF-09
Specific against Human
Isotype IgG2a
ECLASS 5.1 32160702
ECLASS 6.1 32160702
ECLASS 8.0 32160702
ECLASS 9.0 32160702
ECLASS 10.0.1 42030590
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products NF-M, neurofilament, NEF3, NFM, neurofilament triplet M protein, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)
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Concentration
0.5 mg/ml
Description
NF-M is an abundant, stable cytoplasmic protein located in neuronal cells in large axons frequently used as a cell type specific marker. NF-M is modified by glycosylation and phosphorylation. The NF-M protein shares a high degree of structural and sequence homology with the NF-L and NF-H subunits, especially in the coiled-coil core domain. NF-M and NF-H form flexible extensions linking the neurofilament proteins to each other and other cytoplasmic proteins. The NF-09 monoclonal antibody recognizes the highly conserved NF-M protein (phosphorylation and non-phosphorylated form) in all species.The NF-09 antibody has been reported to be useful for Western blot, immunohistochemistry using formalin-fixed paraffin-embedded tissues, and immunofluorescence staining.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Immunogen
Pellet of pig brain cold stable proteins after microtubule depolymerization
Reconstitution
Each lot of this antibody is quality control tested by Western blot. For Western blot, suggested working dilution(s): Use 1 ul per 2 ml antibody dilution buffer for each mini-gel. For IHC, use a 10 ug/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Storage and Stability
Upon receipt, store undiluted at 4C C.
Specificity
Provides flexible extension (along with NF-H) to link neurofilaments to each other and to other cytoplasmic proteins

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 ug
Lieferbar: In stock
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