RAPAd® Universal Adenoviral Expression System

Blue Ice

Engineered to produce 100- to 10, 000-fold fewer wild type plaques than most other expression systems
Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
Promoterless shuttle vector allows you to clone the optimal promoter with your gene of interest
Cloning capacity of shuttle vector = 7.5 kb

Viral Expression

Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. The RAPAd® Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest to maximize gene expression.