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OxiSelect™ Hydrogen Peroxide / Peroxidase Assay Kit (Fluorometric)

OxiSelect™ Hydrogen Peroxide / Peroxidase Assay Kit (Fluorometric)

ArtNr	STA-344
Hersteller	Cell Biolabs
Menge	500 assays
Kategorie	Cell Biology Cell Status Cell Metabolism Oxidative Stress Other Assays & Kits Other Kits
Typ	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Lieferbar
Manufactuers Category	Kit
Manufacturers Product Classification	Oxidative Stress/ Damage->Oxidase/Peroxidase Activity Assays->Hydrogen Peroxide and Peroxidase Assays
Shipping Temp	Room Temp
Storage Temp	Upon receipt, aliquot and store the ADHP probe, HRP at -20ºC. Avoid multiple freeze/thaw cycles. Store the remaining kit components at 4ºC. Protect ADHP from light.
Detection Method	Fluorometric
Product Description	The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
Additional Information	Detect hydrogen peroxide concentrations as low as 50 nM Suitable for use with cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine, or other biological fluids Simple assay protocol provides results in 30-90 minutes, depending on sample type
LMO Content	None

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