ArtNr |
BOS-PROTP23497 |
Hersteller |
Boster
|
Menge |
20ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Solution |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Nuclear autoantigen Sp-100;Nuclear dot-associated Sp100 protein;Speckled 100 kDa;SP100; |
Lieferbar |
|
Contents |
Sp100 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. |
Description |
Recombinant Sp100 antigen is a full-length cDNA coding having a molecular mass of 54, 662 Dalton (pH 5.0).Shows a 79kDa band on SDS-PAGE. Sp100 protein is fused to a hexa-histidine purification tag. |
Gene name |
SP100 |
Protein function |
Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53- mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation. |
Purification |
Greater than 80% as determined by SDS-PAGE. |
Shipping |
Shipped with wet ice |
Short description |
Recombinant Sp100 antigen is a full-length cDNA coding having a molecular mass of 54, 662 Dalton (pH 5.0).Shows a 79kDa band on SDS-PAGE. Sp100 protein is fused to a hexa-histidine purification tag. |
Storage |
Store in -20° C for long term storage. After reconstitution, store in 4° C for short term usage within a few days. Avoid freeze-thaw cycles. |
Subcellular localization |
Nucleus. Nucleus, PML body. Cytoplasm. Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon- dependent. Accumulates in the cytoplasm upon FAS activation. |
Tissue specificity |
Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines. |
Format Information |
Sterile filtered colorless solution. |
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