Sample Data |
Concentration (pg/ml) |
Manufacturer - Specificity |
Natural and recombinant rat Endothelin |
Intra Assay Consistency & Inter Assay Consistency |
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample |
Reproducibility |
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|
Sample 1 |
Capture antibody and Detection antibody |
monoclonal antibody from rat|monoclonal antibody from mouse |
Kit Components |
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4 |
Standard |
1 ng/tube |
Anticoagulant |
heparin or EDTA |
Background |
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease. |
Immunogen |
A peptide reflecting the target protein in human. Sequence: C53-W73 |
Material Required But Not Provided |
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. |
Manufacturer - Research Category |
Cancer, Cancer Metabolism, Cardiovascular, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction, Vasculature |
Protein Name |
Endothelin-1 |
Protein Function |
Endothelins are endothelium-derived vasoconstrictor peptides. |
Subcellular Localization |
Secreted. |
Description |
Rat Endothelin 1/EDN1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Edn1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.
Menge:
96 wells/kit, with removable strips.
Lieferbar:
In stock

lieferbar
Pünktliche Lieferung zu Ihrem Wunschtermin und bei jeder 5. Bestellung 15% sparen.
Schließen
-
Bitte warten Sie, während dieser Artikel in den Warenkorb gelegt wird.
-
-
| |
|