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Mouse Endostatin ELISA Kit PicoKine®

Mouse Endostatin ELISA Kit PicoKine®

Hersteller	Boster
Kategorie	Oncology Cell Biology ELISA Cell Stainings ECM ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer
Typ	Elisa-Kit
Specific against	Mouse
Applikationen	ELISA
Menge	96 wells/kit, with removable strips.
ArtNr	BOS-EK1376
eClass 6.1	32160605
eClass 9.0	32160605
Lieferbar
Alias	Collagen alpha-1 (XVIII) chain;Endostatin;Col18a1;
Manufacturers Product Category	PicoKine® ELISA Kits
Manufacturers Research Category	Angiogenesis, Cancer, Cardiovascular, Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction
Short Description	Mouse Endostatin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Description	Mouse Endostatin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Background	Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Gene Name	COL18A1
Application Details	Dilution ratio of 1:50, concentration in serum and plasma is around 50 ng/ml.
Immunogen	Expression system for standard: NS0; Immunogen sequence: H1591-K1774
Cross-reactivity	There is no detectable cross-reactivity with other relevant proteins.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Capture antibody\|Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Anticoagulant	heparin or EDTA
Gene Full Name	Collagen alpha-1(XVIII) chain
Molecular Weight	182172 MW
Protein Function	Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Subcellular Localization	Secreted, extracellular space, extracellular matrix.
Tissue Specificity	Expressed in liver, kidney, lung, skeletal muscle and testis..
Protein Name	Collagen alpha-1(XVIII) chain
Sequence Similarities	Belongs to the multiplexin collagen family.

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