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Monkey primate IGF-2 PicoKine™ ELISA Kit

Monkey primate IGF-2 PicoKine™ ELISA Kit

ArtNr	BOS-EK0379-PR
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Breast Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Monkey
Sensitivity	<2pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Insulin-like growth factor II;IGF-II;Somatomedin-A;T3M-11-derived growth factor;Insulin-like growth factor II;Insulin-like growth factor II Ala-25 Del;Preptin;IGF2;PP1446;
Lieferbar
Anticoagulant	heparin or EDTA
Assay procedure step1	4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml
Background	Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. The monkey primate IGF-II gene is located on chromosome 11. The standard product used in this kit is recombinant monkey primate IGF-2, consisting of 67 amino acids in mature form with the molecular mass of 7.5KDa. IGF-1 and IGF-2 share high homology.
Components needed	Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Cross reactivity	There is no detectable cross-reactivity with IGF-1.
Description	Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate IGF-2
Gene full name	Insulin-like growth factor II
Gene name	IGF2
Immunogen	Expression system for standard: E.coli; Immunogen sequence: A25-E91
Intra inter assay cv	\|Intra-Assay Precision\|Inter-Assay Precision Sample\|1\|2\|3\|1\|2\|3 n\|16\|16\|16\|24\|24\|24 Mean(pg/ml)\|648\|1657\|2635\|759\|1830\|3176 Standard deviation\|26.6\|81.2\|152.83\|47.82\|122.6\|238.2 CV(%)\|4.1\|4.9\|5.8\|6.3\|6.7\|7.5
Kit components	10ng/tube
MW	20140 MW
Principle	monoclonal antibody from mouse\|polyclonal antibody from goat
Protein function	The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Protein name	Insulin-like growth factor II
Research category	\|signal transduction\|growth factors/hormones\|insulin / insulin-like\| cancer\|growth factors\|insulin and insulin-like\| developmental biology\|post embryonic development\|growth hormones
Sample data	Concentration(pg/ml)\|0\|62.5\|125\|250\|500\|1000\|2000\|4000 O.D.\|0.136\|0.158\|0.179\|0.200\|0.298\|0.612\|1.166\|1.721
Sample type	cell culture supernates, serum and plasma(heparin, EDTA).
Sequence similarities	Belongs to the insulin family.
Storage	Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Subcellular localization	Secreted.
Tmb Incubation time	25-30min

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