Human ACE2 PicoKine ELISA Kit

AngiotensinI-converting enzyme 2(ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

For quantitative detection of human ACE2 in cell culture supernates, serum and plasma(heparin).

ACE2

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|163|424|1746|176|432|1911
Standard deviation|7|16.96|115.23|9.32|18.14|145.23
CV(%)|4.3%|4%|6.6%|5.3%|4.2%|7.6%

Boster’s human ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE2 has been precoated onto 96-well plates. Standards(NSO, Q18-S740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ACE2 amount of sample captured in plate.

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000
O.D.|0.154|0.208|0.258|0.347|0.538|0.885|1.440|2.205

15-20