ArtNr |
BOS-EK0305 |
Hersteller |
Boster
|
Menge |
96 wells/kit, with removable strips. |
Kategorie |
|
Typ |
Elisa-Kit |
Applikationen |
ELISA |
Specific against |
Human |
Sensitivity |
<12 pg/ml |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5; |
Lieferbar |
|
Manufacturers Product Category |
PicoKine® ELISA Kits |
Manufacturers Research Category |
RNA Processing, Signal Transduction, Translation |
Short Description |
Human Angiogenin/ANG ELISA Kit PicoKine® (96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. |
Description |
Human Angiogenin/ANG ELISA Kit PicoKine® (96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. |
Background |
Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11. |
Gene Name |
ANG |
Application Details |
Dilution ratio of 1:200, concentration in serum and plasma is 156-524 ng/ml. |
Immunogen |
Expression system for standard: E.coli; Immunogen sequence: Q25-P147 |
Cross-reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Capture antibody|Detection antibody |
monoclonal antibody from mouse|polyclonal antibody from goat |
Storage |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
Anticoagulant |
heparin or EDTA |
Gene Full Name |
Angiogenin |
Molecular Weight |
16550 MW |
Protein Function |
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.. |
Subcellular Localization |
Nucleus. Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA. |
Tissue Specificity |
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.. |
Protein Name |
Angiogenin |
Sequence Similarities |
Belongs to the pancreatic ribonuclease family. |
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