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Horse equine TGF-Beta 2 ELISA Kit PicoKine®

Horse equine TGF-Beta 2 ELISA Kit PicoKine®

ArtNr

BOS-EK0981-EQ

Hersteller

Boster

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Horse (Equine)

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Transforming growth factor beta-2;TGF-beta-2;BSC-1 cell growth inhibitor;Cetermin;Glioblastoma-derived T-cell suppressor factor;G-TSF;Polyergin;Latency-associated peptide;LAP;TGFB2;

Lieferbar

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

47748 MW

Manufacturer - Gene Name

TGFB2

Gene Full Name

Transforming growth factor beta-2

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Sample Data

Concentration (pg/ml)

Intra Assay Consistency & Inter Assay Consistency

	Intra-Assay Precision			Inter-Assay Precision
Sample
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or citrate
Activating Reagent	TGF beta 2 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 2. Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.
Sample Activation Procedure	Cell culture supernatants: add activating reagent pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6. Serum, plasma(heparin, EDTA or citrate): add activating reagent pro rata, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 2. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.
Background	Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured horse equine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
Immunogen	Expression system for standard: NS0; Immunogen sequence: A303-S414
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Metabolism, Metabolism Processes, Organogenesis, Pathways and Processes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
Protein Name	Transforming growth factor beta-2
Protein Function	TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Subcellular Localization	Secreted.
Description	Horse equine TGF-Beta 2 ELISA Kit PicoKine® (96 Tests). Quantitate Horse TGFB2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sequence Similarities	Belongs to the TGF-beta family.

Horse equine TGF-Beta 2 ELISA Kit PicoKine®

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK0981-EQ.pdf

BOS-EK0981-EQ-datasheet.pdf

BOS-EK0981-EQ-safety-datasheet.pdf