ArtNr |
BOS-P30441 |
Hersteller |
Boster
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
BRAF;BRAF1;RAFB1;Serine/threonine-protein kinase B-raf;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Short Description |
Boster Bio Anti-Phospho B-RAF (Thr599) Antibody (Catalog# P30441). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Phospho B-RAF (Thr599) Antibody (Catalog# P30441). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat. |
Gene Name |
BRAF |
Clonality |
Polyclonal |
Concentration |
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Contents |
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Application Details |
Western Blot, 1:500-2000 Immunohistochemistry, 1:100 - 1:300 ELISA, 1:40000 Not yet tested in other applications. |
Immunogen |
A peptide derived from human B-RAF around the phosphorylation site of Thr599. Immunogen sequence location: 571-620 |
Purification |
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Storage |
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Gene Full Name |
Serine/threonine-protein kinase B-raf |
Molecular Weight |
84437 MW |
Protein Function |
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. |
Subcellular Localization |
Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. |
Tissue Specificity |
Brain and testis. |
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