ArtNr |
BOS-M04320 |
Hersteller |
Boster
|
Menge |
100ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
FC, IF, IHC |
Clon |
TS106 + TMS715 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG1 Kappa |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Thymidylate synthase;TS;TSase;2.1.1.45;TYMS;TS;OK/SW-cl.29; |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Monoclonal Antibodies |
Manufacturers Research Category |
DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways and Processes |
Short Description |
Boster Bio Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (Catalog # M04320). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (Catalog # M04320). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human. |
Background |
This monoclonal antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5, 10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas. |
Gene Name |
TYMS |
Clonality |
Monoclonal |
Concentration |
Purified antibody with BSA and azide at 200ug/ml |
Contents |
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml. |
Application Details |
Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. |
Immunogen |
Recombinant human thymidylate synthase (TS106 & TMS715) |
Purification |
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. |
Storage |
Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Gene Full Name |
Thymidylate synthase |
Molecular Weight |
35716 MW |
Protein Function |
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.. |
Subcellular Localization |
Cytoplasmic and Nuclear |
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