ArtNr |
BOS-A08481 |
Hersteller |
Boster
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody |
Format |
Liquid |
Applikationen |
WB, IHC |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PARC, p53 associated parkin like cytoplasmic protein, RP3 330M21.2, UbcH 7 associated protein 1 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Short Description |
Boster Bio Anti-PARC CUL9 Antibody (Catalog # A08481). Tested in IHC, WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-PARC CUL9 Antibody (Catalog # A08481). Tested in IHC, WB applications. This antibody reacts with Human. |
Background |
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney. |
Gene Name |
CUL9 |
Clonality |
Polyclonal |
Concentration |
85 mg/mL by Refractometry |
Contents |
0.01% (w/v) Sodium Azide |
Application Details |
ELISA: 1:2, 000 - 1:10, 000 IHC: User optimized IP: User optimized WB: 1:500 - 1:1, 000 |
Application Notes |
Anti-PARC reacts with human and mouse PARC by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). To date co-immunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Researchers should determine optimal titers for other applications. |
Immunogen |
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH. |
Purification |
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse PARC. Cross-reactivity with PARC from other sources is not known. |
Storage |
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
Format Information |
Liquid (sterile filtered) |
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