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Anti-PRMT1 Antibody Picoband®

Anti-PRMT1 Antibody Picoband®

ArtNr	BOS-A01417-3
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Antibodies Flow Cytometry Western Blot ELISA Primary Antibodies
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, FC, IHC, ELISA
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Citations	1. Scott HS, Antonarakis SE, Lalioti MD, Rossier C, Silver PA, Henry MF (June 1998). "Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2)". Genomics. 48 (3): 330–40. 2. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. 3. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4; PRMT1; HMT2, HRMT1L2, IR1B4
Lieferbar
Specificity	No cross reactivity with other proteins.
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	42-45 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.25-0.5?g/ml, Human Immunohistochemistry(Paraffin-embedded Section), 0.5-1 ?g/ml, Human, By Heat Flow Cytometry (Fixed), 1-3?g/1x10⁶ cells, Human ELISA, 0.1-0.5?g/ml, -
Manufacturer - Gene Name	PRMT1
Gene Full Name	protein arginine methyltransferase 1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Protein arginine N-methyltransferase 1?is an?enzyme?that in humans is encoded by the?PRMT1?gene. It is mapped to 19q13.33. This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Immunogen	E.coli-derived human PRMT1 recombinant protein (Position: M11-K145).
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, NK Cells, Stem Cells
Protein Function	Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:10749851, PubMed:16879614, PubMed:26876602). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
Subcellular Localization	Apical cell membrane.
Description	Boster Bio Anti-PRMT1 Antibody Picoband® catalog # A01417-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Widely expressed (PubMed:11097842). Expressed strongly in colorectal cancer cells (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (PubMed:28040436).

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BOS-A01417-3-datasheet.pdf

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