ArtNr |
BOS-MA1071 |
Hersteller |
Boster
|
Menge |
100 ug/vial |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Lyophilized |
Applikationen |
WB, IHC |
Clon |
N52 |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
Isotype |
IgG1 |
Citations |
1. Lees, J. F.; Shneidman, P. S.; Skuntz, S. F.; Carden, M. J.; Lazzarini, R. A. : The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. EMBO J. 7: 1947-1955, 1988. <br/>2. Brownlees, J.; Ackerley, S.; Grierson, A. J.; Jacobsen, N. J. O.; Shea, K.; Anderton, B. H.; Leigh, P. N.; Shaw, C. E.; Miller, C. C. J. : Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport. Hum. Molec. Genet. 11: 2837-2844, 2002.<br/> |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;Nefh;Nfh; |
Similar products |
Neurofilament triplet H protein, 200 kDa neurofilament protein, KIAA0845, NFH |
Lieferbar |
|
Specificity |
No cross reactivity with other proteins. |
Manufacturers Product Category |
Primary Antibodies, Mouse Monoclonal Antibodies |
Manufacturers Research Category |
Alzheimer's Disease, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction |
Short Description |
Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT). |
Gene Name |
NEFH |
Application Details |
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, - Western blot, 0.5ml, Human, mouse, rat |
Application Notes |
Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Monoclonal |
Concentration |
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
Contents |
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
Immunogen |
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. |
Purification |
Ascites |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
Gene Full Name |
Neurofilament heavy polypeptide |
Molecular Weight |
115378 MW |
Protein Function |
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. |
Protein Name |
Neurofilament heavy polypeptide |
Sequence Similarities |
Belongs to the intermediate filament family. |
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