ArtNr |
BOS-A01595-1 |
Hersteller |
Boster
|
Menge |
100 ug/vial |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Lyophilized |
Applikationen |
WB, FC, IHC, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Citations |
1. Broix, L., Jagline, H., Ivanova, E. L., Schmucker, S., Drouot, N., Clayton-Smith, J., Pagnamenta, A. T., Metcalfe, K. A., Isidor, B., Louvier, U. W., Poduri, A., Taylor, J. C., and 28 others.Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia. Nature Genet. 48: 1349-1358, 2016.<br/>2. Dunn, D. M., Ishigami, T., Pankow, J., von Niederhausern, A., Alder, J., Hunt, S. C., Leppert, M. F., Lalouel, J.-M., Weiss, R. B. Common variant of human NEDD4L activates a cryptic splice site to form a frameshifted transcript. J. Hum. Genet. 47: 665-676, 2002. |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; NEDD4.2; Nedd4-2; NEDD4L; KIAA0439; NEDL3 |
Lieferbar |
|
Specificity |
No cross reactivity with other proteins. |
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Cell Biology, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin E3 Enzymes |
Short Description |
Boster Bio Anti-NEDD4-2/NEDD4L Antibody Picoband™ catalog # A01595-1. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-NEDD4-2/NEDD4L Antibody Picoband™ catalog # A01595-1. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Neural precursor cell expressed developmentally downregulated gene 4-like (NEDD4L) or NEDD4-2 (NEDD4-2) is an enzyme (ubiquitin ligase) of the NEDD4 family. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Gene Name |
NEDD4L |
Application Details |
Western blot, 0.1-0.5μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E. coli-derived human NEDD4-2 recombinant protein (Position: N703-D975). |
Cross-reactivity |
No cross-reactivity with other proteins. |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase |
Molecular Weight |
130 kDa |
Protein Function |
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, KCNH2, EAAT1 or CLC5 (PubMed:26363003). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Involved in the regulation of TOR signaling (PubMed:27694961). |
Subcellular Localization |
Cytoplasm. |
Tissue Specificity |
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003). |
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