Anti-HEXA Antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

Boster Bio Anti-HEXA Antibody catalog # PA1787-2. Tested in WB applications. This antibody reacts with Human.

HEXA (hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

Western blot, 0.1-0.5μg/ml, Human

Monkey

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA, different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.

No cross-reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

60703 MW

Lysosome.

Belongs to the glycosyl hydrolase 20 family.