CUL5 polyclonal antibody

Q93034

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cul-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/Cul-1/F-box protein complex) consists of Skp1 associating with both Cul-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cul-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

CUL5 pAb detects endogenous levels of CUL5 protein.

Immunofluorescence analysis of U2OS cells, using CUL5 polyclonal antibody

For research use only, not for use in diagnostic procedure.

Recombinant full length Human CUL5.