Histone H4 (aDi-Methyl R3) polyclonal antibody

P62805

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Histone H4 (aDi-Methyl R3) pAb detects endogenous levels of histone H4 only when asymmetric Di-Methyl at Arg3.

Dot-blot analysis of all sorts of methylation peptides using Histone H4 (aDi-Methyl R3) antibody.

ca. 11 kDa

H4R3me2a, H4R3me2