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SPARC polyclonal antibody

SPARC polyclonal antibody

ArtNr	BS6738-100
Hersteller	Bioworld Technology, Inc
Menge	100ul
Kategorie	Neuroscience Neural Adhesion Molecules Antibodies Immunohistochemistry Western Blot Primary Antibodies
Typ	Antibody Polyclonal
Applikationen	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Similar products	SPARC
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Swiss-Prot	P09486
Application all	WB: 1:500ca.1:2000 IHC: 1:50ca.1:200
Background	SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Product	1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	SPARC pAb detects endogenous levels of SPARC protein.
WB	Western blot analysis of SPARC polyclonal antibody
IHC	Immunohistochemistry (IHC) analysis of SPARC polyclonal antibody in paraffin-embedded Rat Kidney tissue.
BiowMW	ca. 35 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Immunogen	Recombinant full length Human SPARC.

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