Hersteller |
Bioworld Technology, Inc
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applikationen |
WB |
Menge |
50ul |
Host |
Rabbit |
ArtNr |
BS60496-50 |
Targets |
CD150;;SH2D1A |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Alias |
SH2 domain-containing protein 1A Alternative name(s): Duncan disease SH2-protein Signaling lymphocytic activation molecule-associated protein Short name: SLAM-associated protein T-cell signal transduction molecule SAP Gene namesi Name:SH2D1A, DSHP, SAP |
Swiss-Prot |
O60880 |
Application all |
WB: 1:500ca.1:1000 |
Background |
SH2D1A, also SH2 domain protein 1A, SAP and CD150/SLAM (signaling lymphocyte activation molecule)-associated protein, influences signaling pathways involving SLAM molecules at the interface between T and B cells. SH2D1A modulates SLAM by blocking the recruitment of tyrosine phosphatase SHP2 to the phosphorylated cytoplasmic domain of SLAM. SLAM activation mediates expansion of activated T cells during immune responses, induces production of interferon- and changes the functional profile of subsets of T cells. SH2D1A is a hydrophilic, 128 amino acid protein that is 96% homologous to the mouse protein in both SH2 and tail domains. SH2D1A is present in all major subsets of T cells, including CD4+, CD45RO+, CD45RA+ and CD8+, but not in B cells. SH2D1A can interact via an SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of cell-surface receptors SLAM (CD150), CD84, CD229 (LY9) and CD244 (2B4). |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
SH2D1A pAb detects endogenous levels of SH2D1A protein. |
WB |
Western blot (WB) analysis of SH2D1A pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate |
BiowMW |
ca. 14 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
SH2 domain-containing protein 1A Alternative name(s): Duncan disease SH2-protein Signaling lymphocytic activation molecule-associated protein Short name: SLAM-associated protein T-cell signal transduction molecule SAP Gene namesi Name:SH2D1A, DSHP, SAP |
Immunogen |
A synthetic peptide corresponding to residues in Human SH2D1A. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.