Hersteller |
Bioworld Technology, Inc
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applikationen |
WB, IF, IHC |
Menge |
100ul |
Host |
Rabbit |
ArtNr |
BS4263-100 |
Targets |
CBL ;;UBE2K |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Alias |
E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL, CBL, CBL2, RNF55 |
Swiss-Prot |
P22681 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL , Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias, c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4, 11) or t(11, 14) abnormalities. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-Cbl (Y774) pAb detects endogenous levels of Cbl only when phosphorylated at Tyr774. |
WB |
Western blot (WB) analysis of p-Cbl (Y774) pAb at 1:500 dilution Lane1:Jurkat cell lysate Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate |
IHC |
Immunohistochemistry (IHC) analysis of p-Cbl (Y774) pAb in paraffin-embedded human breast carcinoma tissue. |
BiowMW |
ca. 100, 120kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL, CBL, CBL2, RNF55 |
Immunogen |
Synthetic phosphopeptide derived from human Cbl around the phosphorylation site of Tyrosine 774. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.