ArtNr |
BS4135-100 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IP, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
p65, p65 NF kappaB, p65 NFkB, RELA, NFKB P65, Transcription Factor p65, Nuclear factor NF-kappa-B p65 subunit, NFkB-p65, NFKB3 |
Similar products |
NFkB-p65 |
Lieferbar |
|
Swiss-Prot |
Q04206 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IP: 1:50ca.1:200 |
Background |
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-NFkappaB-p65 (S276) pAb detects endogenous levels of NFkappaB-p65 protein only when phosphorylated at Ser276. |
WB |
Western blot (WB) analysis of p-NFkB-p65 (S276) pAb at 1:500 dilution Lane1:Hela cell lysate treated with TNFa Lane2:Mouse kidney tissue lysate Lane3:PC12 cell lysate treated with UV |
IHC |
Immunohistochemistry (IHC) analyzes of p-NFkB-65 (S468) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 60, 75 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
p65, p65 NF kappaB, p65 NFkB, RELA, NFKB P65, Transcription Factor p65, Nuclear factor NF-kappa-B p65 subunit, NFkB-p65, NFKB3 |
Immunogen |
Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 276. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.