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GDF-9 (E322) polyclonal antibody

GDF-9 (E322) polyclonal antibody

ArtNr	BS2929-100
Hersteller	Bioworld Technology, Inc
Menge	100ul
Kategorie	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Typ	Antibody Polyclonal
Applikationen	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GDF9, GDF-9, Growth/differentiation factor 9
Similar products	GDF-9
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Swiss-Prot	O60383
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200
Background	GDF 9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF 9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	GDF-9 (E322) pAb detects endogenous levels of GDF-9 protein.
WB	Western blot (WB) analysis of GDF-9 (E322) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate
IHC	Immunohistochemistry (IHC) analyzes of GDF-9 (E322) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
BiowMW	ca. 51 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	GDF9, GDF-9, Growth/differentiation factor 9
Immunogen	Synthetic peptide, corresponding to amino acids 301-350 of Human GDF-9.

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