ArtNr |
BS2598-50 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
50ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Prostaglandin E2 receptor EP2 subtype, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER2 |
Similar products |
EP2 |
Lieferbar |
|
Swiss-Prot |
P43116 |
Application all |
WB: 1:500ca.1:1000 IF: 1:50ca.1:200 |
Background |
Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism. Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors, designated EP1, EP2, EP3 and EP4. The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney. EP1, which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers. EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase. EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit salt-sensitive hypertension, which suggests a role for EP2 in salt excretion. EP3 is expressed in renal vessels, thick ascending limb and collecting duct. EP3 has at least 6 alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
EP2 (Q299) pAb detects endogenous levels of EP2 protein. |
WB |
Western blot (WB) analysis of EP2 (Q299) pAb at 1:500 dilution Lane1:A549 cell lysate Lane2:Mouse spleen tissue lysate Lane3:Rat spleen tissue lysate |
BiowMW |
ca. 40 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Prostaglandin E2 receptor EP2 subtype, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER2 |
Immunogen |
Synthetic peptide, corresponding to amino acids 280-330 of Human EP2. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.