ArtNr |
BS2282-50 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
50ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation |
Similar products |
TFIIA-alpha |
Lieferbar |
|
Swiss-Prot |
P52655 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-alpha, TFIIA-beta and TFIIA-gamma, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
TFIIA-alpha (E311) pAb detects endogenous levels of TFIIA-alpha protein. |
WB |
Western blot (WB) analysis of TFIIA-alpha (E311) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of TFIIA-alpha (E311) pAb in paraffin-embedded human breast carcinoma tissue at 1:50. |
BiowMW |
ca. 42 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation factor IIA beta chain, TFIIA p19 subunit, GTF2A1, TF2A1 |
Immunogen |
Synthetic peptide, corresponding to amino acids 300-350 of Human TFIIA-alpha. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.