14-3-3 zeta (W228) polyclonal antibody

P63104

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

14-3-3 zeta (W228) pAb detects endogenous levels of 14-3-3 protein zeta/delta.

Immunohistochemistry (IHC) analyzes of 14-3-3 zeta (W228) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

For research use only, not for use in diagnostic procedure.

Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 zeta.