ArtNr |
BS1826-50 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
50ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ, 14-3-3 protein zeta, 14-3-3 protein delta, 14-3-3 zeta, 14-3-3 delta, 1433Z, KCIP1, KCIP 1 |
Similar products |
14-3-3 zeta |
Lieferbar |
|
Swiss-Prot |
P63104 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
14-3-3 zeta (W228) pAb detects endogenous levels of 14-3-3 protein zeta/delta. |
WB |
Western blot (WB) analysis of 14-3-3 zeta (W228) pAb at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Hela cell lysate Lane3:sp2/0 cell lysate Lane4:H9C2 cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of 14-3-3 zeta (W228) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 28 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ, 14-3-3 protein zeta, 14-3-3 protein delta, 14-3-3 zeta, 14-3-3 delta, 1433Z, KCIP1, KCIP 1 |
Immunogen |
Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 zeta. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.