Separase (A795) polyclonal antibody

Q14674

Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, Separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, Separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active Separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Separase (A795) pAb detects endogenous levels of Separase protein.

Immunohistochemistry (IHC) analyzes of Separase (A795) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

For research use only, not for use in diagnostic procedure.

Synthetic peptide, corresponding to amino acids 763-824 of Human Separase.