ArtNr |
BS1648-50 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
50ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
G protein-activated inward rectifier potassium channel 1, GIRK-1, Inward rectifier K(+) channel Kir3.1, Potassium channel, inwardly rectifying subfamily J member 3, KCNJ3, GIRK1 |
Similar products |
KIR3.1 |
Lieferbar |
|
Swiss-Prot |
P48549 |
Application all |
WB: 1:500ca.1:1000 IF: 1:50ca.1:200 |
Background |
G protein-coupled inwardly rectifying potassium channels (KIR3.1 through KIR3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex and thalamus regions of the brain. Also known as GIRK, KIR3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gbgamma subunits from G protein heterotrimers bind to and regulate KIR3 channel activity. Gb3- and Gb4-containing Gbgamma dimers bind directly to cytoplasmic domains of KIR3 proteins and increase the K+ current while Gb5-containing Gbgamma dimers inhibit KIR3 K+ current. KIR3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates KIR3 tyrosine residues, effectively inactivating the KIR3 channels. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
KIR3.1 (F181) pAb detects endogenous levels of KIR3.1 protein. |
WB |
Western blot (WB) analysis of KIR3.1 (F181) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate |
BiowMW |
ca. 55 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
G protein-activated inward rectifier potassium channel 1, GIRK-1, Inward rectifier K(+) channel Kir3.1, Potassium channel, inwardly rectifying subfamily J member 3, KCNJ3, GIRK1 |
Immunogen |
Synthetic peptide, corresponding to amino acids 150-200 of Human KIR3.1. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.