ArtNr |
BS1378-100 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Acetyl-CoA carboxylase 1, Short name=ACC1, ACC-alpha, Biotin carboxylase, ACACA, ACAC, ACC1, ACCA |
Similar products |
ACCalpha |
Lieferbar |
|
Swiss-Prot |
Q13085 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACCalpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCbeta (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCalpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACCalpha, which maps to human chromosome 17, encodes the 265 kDa alpha form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCbeta is homologous to that of ACCalpha except for an additional peptide of about 150 amino acids at the N-terminus. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
ACCalpha (H74) pAb detects endogenous levels of ACCalpha protein. |
WB |
Western blot (WB) analysis of ACCalpha (H74) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of ACCalpha (H74) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 265 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Acetyl-CoA carboxylase 1, Short name=ACC1, ACC-alpha, Biotin carboxylase, ACACA, ACAC, ACC1, ACCA |
Immunogen |
Synthetic peptide, corresponding to amino acids 50-100 of Human ACCalpha. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.