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Chicken antibody to GFR alpha-1: affinity purified

ArtNr C-1659-100
Hersteller Biosensis
Menge 100ug
Kategorie
Typ Antibody
Applikationen WB, ICC
Specific against other
Host Chicken
Isotype IgY
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products RET ligand 1, TGF-beta related neurotrophic factor receptor 1
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Sub category
Neurotrophic Factor Receptors-Sensory Nervous System
products_extra_fields_value
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRα, -1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
products_quantity
200
storage
Store at +4AC upon receipt, DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
purityisotype
Affinity purified
formulation
Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
immunogen
Recombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
conality
Chicken
specificity
Human GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
reconstitution
Store antibody stock solution at +4AC upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ug
Lieferbar: In stock
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