ArtNr |
BM-EKU06249 |
Hersteller |
Biomatik
|
Menge |
96T |
Kategorie |
|
Typ |
Elisa-Kit |
Format |
96 well plate |
Applikationen |
ELISA |
Specific against |
Rat |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Lieferbar |
|
Detection Range |
24.69-2000 pg/mL |
Sensitivity |
8.73 pg/mL |
Sample Type |
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Time |
2 hours |
UniProt ID |
P07808 |
Gene ID |
100912228 |
Gene Name |
Neuropeptide Y |
Gene Aliases |
NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY |
Assay Type |
Competitive |
Detection Method |
Colorimetric |
Shipping Condition |
Blue ice |
Storage Instructions |
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
Precaution of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
Expiry Date |
8 months |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y (NPY). No significant cross-reactivity or interference between Neuropeptide Y (NPY) and analogues was observed. |
Test Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuropeptide Y (NPY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuropeptide Y (NPY) and unlabeled Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific to Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuropeptide Y (NPY) in the sample. |
Assay Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y (NPY) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y (NPY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Restrictions |
For research use only. Not for diagnostic procedures. |
Quality Systems |
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
Research Area |
Metabolic pathway; Endocrinology; Cardiovascular biology; Neuro science; Hepatology; |
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