Mouse PGF2alpha (Prostaglandin F2 Alpha) ELISA Kit

PGF2alpha

15.63-1000 pg/mL

Colorimetric

Competitive

Short term: 4C; Long term: see manual.

6 months

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse PGF2alpha. During the reaction, Mouse PGF2alpha in the sample or standard competes with a fixed amount of Mouse PGF2alpha on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse PGF2alpha in the samples is then determined by comparing the OD of the samples to the standard curve.

The kit is manufactured at ISO 9001 certified facilities.