ArtNr |
BM-EKU11642 |
Hersteller |
Biomatik
|
Menge |
96T |
Kategorie |
|
Typ |
Elisa-Kit |
Applikationen |
CLIA |
Specific against |
Human |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Lieferbar |
|
Format |
96-Well Plate |
Target Name |
Alpha 2-Antiplasmin |
Target Synonyms |
SERPINF2; AAP; A2-PI; API; PLI; A2AP; Alpha-2-Plasmin Inhibitor; Serpin Peptidase Inhibitor Clade F Member 2; Pigment Epithelium Derived Factor |
Sample type |
Plasma |
Detection ra nge |
1.56-100 ng/mL |
Sensitivity |
0.71 ng/mL |
Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha 2-Antiplasmin (a2PI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha 2-Antiplasmin (a2PI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%. |
Detection Method |
Chemiluminescent |
Assay Time |
2 hours, 40min |
Assay Type |
Double-antibody Sandwich |
Shipping Condition |
Ice packs |
Storage |
Short term: 4CC; Long term: see manual. |
Precaution of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. |
Expiry Date |
8 months |
UniProt ID |
n/a |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Alpha 2-Antiplasmin (a2PI). |
Cross Activity |
This assay has high sensitivity and excellent specificity for detection of Alpha 2-Antiplasmin (a2PI). |
Test principle |
The microplate provided in this kit has been pre-coated with an antibody specific to Alpha 2-Antiplasmin (a2PI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha 2-Antiplasmin (a2PI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha 2-Antiplasmin (a2PI) level in the sample or standard.; |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37CC; 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37CC; 4. Aspirate and wash 3 times; 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 6. Aspirate and wash 5 times; 7. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 8. Read RLU value immediately. |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Restrictions |
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals. |
Quality Systems |
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
Quality Assurance |
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.