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Anti-Mouse TCR beta BG Violet 450 Size 25 ug

ArtNr 89612-40-25
Hersteller BioGems
Menge 25 ug
Kategorie
Typ Antibody
Format BG Violet 450
Applikationen FC
Clon H57-597
Specific against Mouse
Host Hamster
Isotype IgG
Konjugat/Tag BG Violet 450
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Description
The H57-597 monoclonal antibody is specific for the beta chain of the mouse T cell Receptor (TCR). The crosslinking induces activation and proliferation of T cells, as a plate-bound or a soluble H57-597, or the plate-bound antibody can induce apoptosis, based on assay conditions. The beta chain of the TCR can combine with the alpha chain of the receptor to produce the alpha-beta TCR, which is expressed by the NKT cells, by the NK1.1+ thymocytes and most of the T cells. The beta chain does not react with the gamma-delta TCR-bearing cells, expressed by a small number of T cells.

This antibody can be used as a phenotypic marker for the TCR beta expressing cells or for the functional purpose of TCR-mediated cell activation or apoptosis.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Research Interest
Adaptive Immunity
Preperation
The product should be stored undiluted at 4C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation
Phosphate-buffered aqueous solution, ≤ 0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References

Kubo, R. T., Born, W., Kappler, J. W., Marrack, P., & Pigeon, M. (1989). Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. The Journal of Immunology, 142(8), 2736-2742.

Gascoigne, N. R. (1990). Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. Journal of Biological Chemistry, 265(16), 9296-9301.

Kruisbeek, A. M., Shevach, E., & Thornton, A. M. (2004). Proliferative assays for T cell function. Current Protocols in Immunology, 3-12.

Saint-Ruf, C., Panigada, M., Azogui, O., Debey, P., von Boehmer, H., & Grassi, F. (2000). Different initiation of pre-TCR and γ δ TCR signalling. Nature, 406(6795), 524-527.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 ug
Lieferbar: In stock
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