Warning |
- The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C.- The fluorescein (FITC) and Cyanine 5 (CY5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3.Store at 2- 8 C.- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20ï‚CC.- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20ï‚CC.- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C.- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
Dilution |
Immunofluorescence: ELISA: FITC conjugate BIOT conjugate R-PE, APC, SPRD, CY5 and R-PE-CY5.5 conjugates AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:2, 000-1:4, 000 1:4, 000-1:8, 000 1:5, 000-1:20, 000 |