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PrecipHen® Immunoprecipitation Reagent

PrecipHen® Immunoprecipitation Reagent

ArtNr	P-1010
Hersteller	Aves Labs
Menge	2 mL
Kategorie	Antibodies Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies
Typ	Reagents
Format	Affinity Purified
Applikationen	IP
Specific against	Chicken (Gallus gallus domesticus)
Host	Caprine
Isotype	IgG
Konjugat/Tag	Agarose
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12000000
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	Immunoprecipitation Reagent
Manufacturer - Targets	Chicken IgY
Country of Origin	United States
Shipping Temperature	Cold Pack
Storage Conditions	Store at 4°C in the dark. Under these conditions, this product should have a shelf life of at least twelve months, provided they remain sterile.
Shipping Method	Overnight Priority
Buffer	Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent)
Quality Control	The capacity of PrecipHen® was determined by measuring the 2.5 mLs of PrecipHen. One milliliter aliquots of chicken IgY (200 µg/mL in TE buffer) were applied to the column and fractions were collected. A similar column of agarose (non-NHS activated) was used for comprison. The concentration of IgY passing through each column was measured, and the total amount of IgY that bound to the column was calculated.
Description	Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with chicken antibodies in immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and protein purification applications.
Manufacturer - Statement	Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with IP, CHiP, and protein purification applications.
Antibody Registry ID (RRID)	AB_2313533
Clonality	Polyclonal
Manufacturer - Specificity	H&L; does not cross-react with mammalian IgGs.
Manufacturer - Form	Affinity Purified
Physical State	Agarose beads in buffer, binding capacity 1.0 mg of chicken IgY per ml bed volume 1.0 mg of chicken IgY per ml bed volume
Expiration	12 months from receipt of product
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes	Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”). Sodium azide was then added to a final concentration of 0.02% (w/v).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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P-1010-datasheet.pdf

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Menge: 2 mL

lieferbar

Lieferung vsl. bis 28.03.2025

Express-Lieferung: 8 Tage sparen

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