Vergleich

RayPlex® Human Cytotoxic T Cell Array 1

ArtNr FAH-CTC-1-480
Hersteller Raybiotech
Menge 480 tests
Kategorie
Typ Array
Specific against other
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Lieferbar
Number of Targets Detected
12
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants
Plasma
Serum
Design Principle
Sandwich-based
Method of Detection
Flow Cytometry
Quantitative/Semi-Quantitative
Quantitative
Solid Support
Fluorescent Microbeads
Gene Symbols
CTLA3 | FAS | FASLG | IFNG | IL10 | IL17A | IL2 | IL4 | IL6 | LTA| TNFSF10
Application Information
Multiplexed Protein Detection
Biomarker Screening
Identifying Key Factors
Confirming Biological Process
Biomarker Validation
Validation of Antibody Array Results
Quantitative Protein Detection
Establishing Normal Range
Kit Components
Ray
Plex™ Multiplex Bead Cocktail1
X Assay Diluent20
X Wash Buffer
Ray
Bio® Serum Diluent
Lyophilized Protein Standard Mix1
X Biotinylated Detection Antibody Cocktail10
X Streptavidin-
P
E
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
Other Materials Required
Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (
P
E) and allophycocyanin (
A
P
C) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-
Well Microplate (
Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (
H
T
S) for 96-well plate reading
Don't have a compatible flow cytometer? Ray
Biotech provides full testing services for Ray
Plex™ bead arrays. Get a quote!
Protocol Outline
Prepare Reagents, Standards, and Samples
Add Ray
Plex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-
Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 480 tests
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 23.08.2024 

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