ArtNr |
YLD8157-100ul |
Hersteller |
YL Biotech
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IHC |
Specific against |
Human, Mouse, Rat, Monkey, Bovine, Pig |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Vimentin |
Lieferbar |
|
Background |
Rabbit polyclonal antibody to Vimentin |
Concentration |
1mg/mL |
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Vimentin. The exact sequence is proprietary. |
WB description |
Western blot analysis of Vimentin expression in Hela (A), Jurkat (B), A549 (C) whole cell lysates. |
IHC description |
Immunohistochemical analysis of Vimentin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
Purification_Method |
The antibody was purified by immunogen affinity chromatography. |
Clonality |
Polyclonal |
Buffer |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution |
WB (1/500 - 1/2000), IH (1/50 - 1/200) |
Storage |
Store at -20℃. Avoid repeated freeze / thaw cycles. |
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