ArtNr |
YLD6167-50ul |
Hersteller |
YL Biotech
|
Menge |
50ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IF, IHC, IC |
Specific against |
Human, Mouse, Rat, Monkey, Bovine, Pig, Carp |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1 |
Lieferbar |
|
Background |
Rabbit polyclonal antibody to PIN1 |
Concentration |
1mg/mL |
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PIN1. The exact sequence is proprietary. |
WB description |
Western blot analysis of PIN1 expression in HepG2 (A), Raji (B) whole cell lysates. |
IHC description |
Immunohistochemical analysis of PIN1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
IF/ICC description |
Immunofluorescent analysis of PIN1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
Purification_Method |
The antibody was purified by immunogen affinity chromatography. |
Clonality |
Polyclonal |
Buffer |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution |
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) |
Storage |
Store at -20℃. Avoid repeated freeze / thaw cycles. |
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