ArtNr |
YLD3722-100ul |
Hersteller |
YL Biotech
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IF, IC |
Specific against |
Human, Rat, Rabbit, Bovine, Pig, Ovine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
BHLHA27; EHAND; Heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND |
Lieferbar |
|
Background |
Rabbit polyclonal antibody to HAND1 |
Concentration |
1mg/mL |
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HAND1. The exact sequence is proprietary. |
WB description |
Western blot analysis of HAND1 expression in HeLa (A), H9C2 (B) whole cell lysates. |
IF/ICC description |
Immunofluorescent analysis of HAND1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
Purification_Method |
The antibody was purified by immunogen affinity chromatography. |
Clonality |
Polyclonal |
Buffer |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution |
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
Storage |
Store at -20℃. Avoid repeated freeze / thaw cycles. |
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