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Rabbit anti-HAGH Antibody

Rabbit anti-HAGH Antibody

ArtNr	YLD3718-50ul
Hersteller	YL Biotech
Menge	50ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Polyclonal
Applikationen	WB, IF, IHC, IC
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GLO2; HAGH1; Hydroxyacylglutathione hydrolase, mitochondrial; Glyoxalase II; Glx II
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Background	Rabbit polyclonal antibody to HAGH
Concentration	1mg/mL
Immunogen	Recombinant full length protein of human HAGH
WB description	Western blot analysis of HAGH expression in HepG2 (A), mouse liver (B), mouse heart (C), rat kidney (D) whole cell lysates.
IHC description	Immunohistochemical analysis of HAGH staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description	Immunofluorescent analysis of HAGH staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution	WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)
Storage	Store at -20℃. Avoid repeated freeze / thaw cycles.

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Menge: 50ul

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Lieferung vsl. bis 13.09.2024

Express-Lieferung: 17 Tage sparen

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