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Rabbit anti-Delta Opioid Receptor (pS363) Antibody

ArtNr YLD2471-100ul
Hersteller YL Biotech
Menge 100ul
Kategorie
Typ Antibody Polyclonal
Applikationen WB, IF, IHC, IC
Specific against Human, Mouse, Rat, Pig
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias OPRD; Delta-type opioid receptor; D-OR-1; DOR-1
Lieferbar
Background
Rabbit polyclonal antibody to Delta Opioid Receptor (pS363)
Concentration
1mg/mL
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Delta Opioid Receptor (pS363). The exact sequence is proprietary.
WB description
Western blot analysis of Delta Opioid Receptor (pS363) expression in human brain (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Delta Opioid Receptor (pS363) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Delta Opioid Receptor (pS363) staining in SKNSH cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Purification_Method
The antibody was purified by immunogen affinity chromatography.
Clonality
Polyclonal
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Storage
Store at -20℃. Avoid repeated freeze / thaw cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ul
Lieferbar: In stock
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