ArtNr |
YLD0639-100ul |
Hersteller |
YL Biotech
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Applikationen |
WB, IF, IP, IHC, IC |
Specific against |
Human, Mouse, Rat, Bovine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36 |
Lieferbar |
|
Background |
Rabbit polyclonal antibody to ATF1 |
Concentration |
1mg/mL |
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATF1. The exact sequence is proprietary. |
WB description |
Western blot analysis of ATF1 expression in HeLa (A), KNRK (B), A431 (C), SP2/0 (D), mouse brain (E), H9C2 (F) whole cell lysates. |
IHC description |
Immunohistochemical analysis of ATF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
IF/ICC description |
Immunofluorescent analysis of ATF1 staining in KNRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
Purification_Method |
The antibody was purified by immunogen affinity chromatography. |
Clonality |
Polyclonal |
Buffer |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution |
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) |
Storage |
Store at -20℃. Avoid repeated freeze / thaw cycles. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.