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Rabbit anti-AS160 (pT642) Antibody

Rabbit anti-AS160 (pT642) Antibody

ArtNr	YLD0602-100ul
Hersteller	YL Biotech
Menge	100ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Polyclonal
Applikationen	WB, IF, IHC, IC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160
Lieferbar
Background	Rabbit polyclonal antibody to AS160 (pT642)
Concentration	1mg/mL
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AS160. The exact sequence is proprietary.
WB description	Western blot analysis of AS160 (pT642) expression in LO2 insulin-treated (A), Raw264.7 insulin-treated (B), PC12 insulin-treated (C), NIH3T3 UV-treated (D) whole cell lysates.
IHC description	Immunohistochemical analysis of AS160 (pT642) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description	Immunofluorescent analysis of AS160 (pT642) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Purification_Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution	WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Storage	Store at -20℃. Avoid repeated freeze / thaw cycles.

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Menge: 100ul

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Lieferung vsl. bis 13.09.2024

Express-Lieferung: 17 Tage sparen

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