Anti-Androgen Receptor antibody (N-Term) (STJ70576)

Pep-ELISA/WB/IHC/IF/ChIP

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:64000.
WB: Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
IHC: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3µg/ml. Cryosections of Mouse Hypothalamus and Amygdala. Recommended dilution range: 1:20, 000.
IF: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

N-Term